Review



cellfectin ii  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Thermo Fisher cellfectin ii
    Cellfectin Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellfectin+ii/cellfectin+ii/pmc10903943__Supplemental_Methods_and_References-25-14-16
    Average 90 stars, based on 1 article reviews
    cellfectin ii - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Generated:

    Article Title: Structural basis of voltage-dependent gating in BK channels.
    Article Snippet: Residues 1 to 1070 of the A. californica Slo1 channel were cloned into the pFastbac vector containing a C-terminal 3 Cprotease site, eGFP. .. P0 baculoviruswas generated using theBac-toBac method (Invitrogen) using Cellfectin II as the transfection reagent (Thermo Fisher, 10362100). .. The QuikChange site-directed mutagenesismethod (Agilent) was used to introduce themutations R196Q (R1), R199Q (R2), R202Q (R3), and F304A into pFastbac-aSlo1 using KOD DNA polymerase (71085, EMD Millipore).

    Transfection:

    Article Title: Structural basis of voltage-dependent gating in BK channels.
    Article Snippet: Residues 1 to 1070 of the A. californica Slo1 channel were cloned into the pFastbac vector containing a C-terminal 3 Cprotease site, eGFP. .. P0 baculoviruswas generated using theBac-toBac method (Invitrogen) using Cellfectin II as the transfection reagent (Thermo Fisher, 10362100). .. The QuikChange site-directed mutagenesismethod (Agilent) was used to introduce themutations R196Q (R1), R199Q (R2), R202Q (R3), and F304A into pFastbac-aSlo1 using KOD DNA polymerase (71085, EMD Millipore).

    Article Title: PSKH1 kinase activity is differentially modulated via allosteric binding of Ca 2+ sensor proteins
    Article Snippet: Sf21 insect cells (Merck) were cultured in Insect-XPRESS (Lonza) media. .. Each bacmid (1μg) was introduced into 0.9 × 106 Sf21 cells by Cellfectin II (Thermo Fisher Scientific) mediated transfection in six-well plates using the Bac-to-Bac protocol (Thermo Fisher Scientific), as detailed elsewhere (7). ..

    Article Title:
    Article Snippet: The insect SF21 cells were grown in the SF900 II medium (Gibco). .. Actively dividing SF21 cells were transfected with EBF1 wt, ΔC, or Y>A bacmids using Cellfectin II (Thermo Fisher). ..

    Article Title: A broadly protective human antibody for GI genogroup noroviruses.
    Article Snippet: Inga Rimkute, Adam S. Olia, Mehin Suleiman, Kamron D. Woods, Tatsiana Bylund, Nicholas C. Morano, Ena S. Tully, Raffaello Verardi, Saran Bao, Margaret H. Beddall, Natthawan Chaimongkol, Mitzi M. Donaldson, Renguang Du, Caitlyn N. M. Dulan, Jason Gorman , Amy R. Henry, Chaim A. Schramm , Stanislav V. Sosnovtsev, Tyler Stephens, John-Paul Todd, Yaroslav Tsybovsky , Daniel C. Douek , Kim Y.. Green, Reda Rawi , Lawrence Shapiro, Tongqing Zhou , Peter D. Kwong & Mario Roederer

    Virus:

    Article Title: Condensin II activation by M18BP1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-CAP-H Novus Biologicals Cat# NBP1-32573 Rabbit anti-CAP-D3 Bethyl Laboratories Cat# A300-601A Rabbit anti-CAP-D3 Bethyl Laboratories Cat# A300-604A Rabbit anti-CAP-G2 Bethyl Laboratories Cat# A300-605A Rabbit anti-CAP-H2 Bethyl Laboratories Cat# A302-275A Mouse anti-CENP-A Invitrogen Cat# MA1-20832 Human anti-CREST Antibodies Incorporated Cat# 15-234 Rabbit anti-H3S10ph Abcam Cat# Ab5176 Mouse anti-Tubulin Sigma Aldrich Cat# T5168 Mouse anti-Tubulin Sigma Aldrich Cat# T9026 Rat anti-M18BP1 Conti et al. 34 N/A Mouse anti-CyclinB1 Santa Cruz Cat# sc-245 Rabbit anti-CyclinB1 Abcam Cat# Ab32053 Mouse anti-PCNA Cell Signalling Technology Cat# 2586S Mouse anti-Histone H3 Abcam Cat# ab10799 Rabbit anti-MCPH1 Cell Signalling Technologies Cat# 4120 Rabbit anti-GFP Musacchio Lab N/A Goat anti-CENP-C Musacchio Lab N/A Bacterial and virus strains RosettaTM(DE3) Competent Cells Novagen Cat#70954 DH10EMBacY Gibco Cat#10361012 Chemicals, peptides, and recombinant proteins Human condensin I-strep Kong et al. 46 N/A Human condensin II-strep Kong et al. 46 N/A Human condensin II ΔCAP-G2 Houlard et al. 19 N/A Human condensin II Qloop-strep Kong et al. 46 N/A CAP-G2 Kong et al. 46 N/A CAP-H2 Kong et al. 46 N/A MBP-MCPH1 1-195 Houlard et al. 19 N/A MBP-MCPH1 196-435 Houlard et al. 19 N/A MBP-MCPH1 1-435 Houlard et al. 19 N/A MBP-M18BP1 873-1132 This study N/A MBP-M18BP1 873-1132-4A This study N/A MBP-M18BP1 983-1045 This study N/A MBP-M18BP1 983-1045-7A This study N/A 5FAM-MCPH1 407-422 GenScript N/A 5FAM-MCPH1 407-422 -phosphoSer417 GenScript N/A Sulfo-SDA (Sulfo-NHS-Diazirine) Sigma-Aldrich Cat803340 Cellfectin II ThermoFisher Cat# 10362100 Benzonase Sigma-Aldrich Cat# E1014 ATP Sigma-Aldrich Cat# A1852 PierceTM Protease Inhibitor Tablets, EDTA-free ThermoFisher Cat# A32965 Terrific Broth ThermoFisher Cat# 22711022 (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 17, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMP-PNP Roche Cat# 10102547001 DTT Thermo Fisher Cat# 10699530 Iodoacetamide Sigma-Aldrich I6125 Trypsin Protease Pierce 90057 Acetonitrile, LC-MS grade Sigma-Aldrich Cat# A955-4 Trifluoroacetic acid (TFA), LC-MS grade Sigma-Aldrich Cat# 302031 DMSO Thermo Fisher Cat# D2650 β-Octylglucoside (beta-glucopyranoside) Sigma-Aldrich Cat# O8001 Tween-20 Sigma-Aldrich Cat# P9416 CDK1-Cyclin B with CKS1 Huis In ’t Veld et al. 58 N/A Fugene6 Promega Cat# E2692 Nocodazole Sigma Cat# m1404 Prolong Gold Antifade Invitrogen Cat# P36930 ProLong Glass Antifade Mountant with NucBlue Invitrogen Cat# P36981 Janelia Fluor HaloTag ligand 646 Promega Cat# GA1121 Doxycycline Sigma-Aldrich Cat# D9891 Puromycin Sigma-Aldrich Cat# P9620 Thymidine Sigma-Aldrich Cat# T1895 MG132 MilliporeSigma Cat# 474790 RO-3306 Merck Millipore Cat# 217699 SNAP-Cell Block New England Biolabs (NEB) Cat# S9106S SNAP-Cell 647 New England Biolabs (NEB) Cat# S9102S Janelia Fluor HaloTag Ligand 646 Promega Cat# GA1121 Phosphatase Inhibitor Cocktail Sigma Cat# P0044 DNase I (RNase-free) Invitrogen (Ambion) Cat# AM2222 DAPI Sigma-Aldrich Cat# D9542 Mowiol EMD Millipore Cat# 475904 Triton X-100 Sigma-Aldrich Cat# T8787 NP-40 Sigma-Aldrich Cat# 74385 Opti-MEM Gibco Cat# 31985070 Critical commercial assays ATPase/GTPase Activity Assay Kit Sigma-Aldrich Cat#MAK-113 Superose 6 Increase Sigma-Aldrich Cat#GE29-0915-98 Superdex 30 Increase 10/300 GL column Cytiva GE29219757 Deposited data Atomic coordinates of the condensin II-M18BP1 complex PDB 9F5W Cryo-EM map of the condensin II-M18BP1 complex EMDB EMD-50201 Mass spec proteomics data PRIDE PXD051556 Mass spec XL-MS data PRIDE PXD051886 Genetic screen This study European Nucleotide Archive accession number PRJEB89880 Experimental models: Cell lines sf9 GIBCO Cat#11496015 High Five cells GIBCO Cat#B85502 hTERT RPE1 ATCC CRL-4000 hTERT RPE1 M18BP1-mNeonGreen-FKBP-V This study N/A HeLa CENP-A-SNAP Flp-In T-REx Pan et al. 28 N/A (Continued on next page) Molecular Cell 85, 1–13.e1–e11, July 17, 2025 e2

    Article Title: A broadly protective human antibody for GI genogroup noroviruses.
    Article Snippet: Inga Rimkute, Adam S. Olia, Mehin Suleiman, Kamron D. Woods, Tatsiana Bylund, Nicholas C. Morano, Ena S. Tully, Raffaello Verardi, Saran Bao, Margaret H. Beddall, Natthawan Chaimongkol, Mitzi M. Donaldson, Renguang Du, Caitlyn N. M. Dulan, Jason Gorman , Amy R. Henry, Chaim A. Schramm , Stanislav V. Sosnovtsev, Tyler Stephens, John-Paul Todd, Yaroslav Tsybovsky , Daniel C. Douek , Kim Y.. Green, Reda Rawi , Lawrence Shapiro, Tongqing Zhou , Peter D. Kwong & Mario Roederer

    Recombinant:

    Article Title: Condensin II activation by M18BP1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-CAP-H Novus Biologicals Cat# NBP1-32573 Rabbit anti-CAP-D3 Bethyl Laboratories Cat# A300-601A Rabbit anti-CAP-D3 Bethyl Laboratories Cat# A300-604A Rabbit anti-CAP-G2 Bethyl Laboratories Cat# A300-605A Rabbit anti-CAP-H2 Bethyl Laboratories Cat# A302-275A Mouse anti-CENP-A Invitrogen Cat# MA1-20832 Human anti-CREST Antibodies Incorporated Cat# 15-234 Rabbit anti-H3S10ph Abcam Cat# Ab5176 Mouse anti-Tubulin Sigma Aldrich Cat# T5168 Mouse anti-Tubulin Sigma Aldrich Cat# T9026 Rat anti-M18BP1 Conti et al. 34 N/A Mouse anti-CyclinB1 Santa Cruz Cat# sc-245 Rabbit anti-CyclinB1 Abcam Cat# Ab32053 Mouse anti-PCNA Cell Signalling Technology Cat# 2586S Mouse anti-Histone H3 Abcam Cat# ab10799 Rabbit anti-MCPH1 Cell Signalling Technologies Cat# 4120 Rabbit anti-GFP Musacchio Lab N/A Goat anti-CENP-C Musacchio Lab N/A Bacterial and virus strains RosettaTM(DE3) Competent Cells Novagen Cat#70954 DH10EMBacY Gibco Cat#10361012 Chemicals, peptides, and recombinant proteins Human condensin I-strep Kong et al. 46 N/A Human condensin II-strep Kong et al. 46 N/A Human condensin II ΔCAP-G2 Houlard et al. 19 N/A Human condensin II Qloop-strep Kong et al. 46 N/A CAP-G2 Kong et al. 46 N/A CAP-H2 Kong et al. 46 N/A MBP-MCPH1 1-195 Houlard et al. 19 N/A MBP-MCPH1 196-435 Houlard et al. 19 N/A MBP-MCPH1 1-435 Houlard et al. 19 N/A MBP-M18BP1 873-1132 This study N/A MBP-M18BP1 873-1132-4A This study N/A MBP-M18BP1 983-1045 This study N/A MBP-M18BP1 983-1045-7A This study N/A 5FAM-MCPH1 407-422 GenScript N/A 5FAM-MCPH1 407-422 -phosphoSer417 GenScript N/A Sulfo-SDA (Sulfo-NHS-Diazirine) Sigma-Aldrich Cat803340 Cellfectin II ThermoFisher Cat# 10362100 Benzonase Sigma-Aldrich Cat# E1014 ATP Sigma-Aldrich Cat# A1852 PierceTM Protease Inhibitor Tablets, EDTA-free ThermoFisher Cat# A32965 Terrific Broth ThermoFisher Cat# 22711022 (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 17, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMP-PNP Roche Cat# 10102547001 DTT Thermo Fisher Cat# 10699530 Iodoacetamide Sigma-Aldrich I6125 Trypsin Protease Pierce 90057 Acetonitrile, LC-MS grade Sigma-Aldrich Cat# A955-4 Trifluoroacetic acid (TFA), LC-MS grade Sigma-Aldrich Cat# 302031 DMSO Thermo Fisher Cat# D2650 β-Octylglucoside (beta-glucopyranoside) Sigma-Aldrich Cat# O8001 Tween-20 Sigma-Aldrich Cat# P9416 CDK1-Cyclin B with CKS1 Huis In ’t Veld et al. 58 N/A Fugene6 Promega Cat# E2692 Nocodazole Sigma Cat# m1404 Prolong Gold Antifade Invitrogen Cat# P36930 ProLong Glass Antifade Mountant with NucBlue Invitrogen Cat# P36981 Janelia Fluor HaloTag ligand 646 Promega Cat# GA1121 Doxycycline Sigma-Aldrich Cat# D9891 Puromycin Sigma-Aldrich Cat# P9620 Thymidine Sigma-Aldrich Cat# T1895 MG132 MilliporeSigma Cat# 474790 RO-3306 Merck Millipore Cat# 217699 SNAP-Cell Block New England Biolabs (NEB) Cat# S9106S SNAP-Cell 647 New England Biolabs (NEB) Cat# S9102S Janelia Fluor HaloTag Ligand 646 Promega Cat# GA1121 Phosphatase Inhibitor Cocktail Sigma Cat# P0044 DNase I (RNase-free) Invitrogen (Ambion) Cat# AM2222 DAPI Sigma-Aldrich Cat# D9542 Mowiol EMD Millipore Cat# 475904 Triton X-100 Sigma-Aldrich Cat# T8787 NP-40 Sigma-Aldrich Cat# 74385 Opti-MEM Gibco Cat# 31985070 Critical commercial assays ATPase/GTPase Activity Assay Kit Sigma-Aldrich Cat#MAK-113 Superose 6 Increase Sigma-Aldrich Cat#GE29-0915-98 Superdex 30 Increase 10/300 GL column Cytiva GE29219757 Deposited data Atomic coordinates of the condensin II-M18BP1 complex PDB 9F5W Cryo-EM map of the condensin II-M18BP1 complex EMDB EMD-50201 Mass spec proteomics data PRIDE PXD051556 Mass spec XL-MS data PRIDE PXD051886 Genetic screen This study European Nucleotide Archive accession number PRJEB89880 Experimental models: Cell lines sf9 GIBCO Cat#11496015 High Five cells GIBCO Cat#B85502 hTERT RPE1 ATCC CRL-4000 hTERT RPE1 M18BP1-mNeonGreen-FKBP-V This study N/A HeLa CENP-A-SNAP Flp-In T-REx Pan et al. 28 N/A (Continued on next page) Molecular Cell 85, 1–13.e1–e11, July 17, 2025 e2

    Protease Inhibitor:

    Article Title: Condensin II activation by M18BP1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-CAP-H Novus Biologicals Cat# NBP1-32573 Rabbit anti-CAP-D3 Bethyl Laboratories Cat# A300-601A Rabbit anti-CAP-D3 Bethyl Laboratories Cat# A300-604A Rabbit anti-CAP-G2 Bethyl Laboratories Cat# A300-605A Rabbit anti-CAP-H2 Bethyl Laboratories Cat# A302-275A Mouse anti-CENP-A Invitrogen Cat# MA1-20832 Human anti-CREST Antibodies Incorporated Cat# 15-234 Rabbit anti-H3S10ph Abcam Cat# Ab5176 Mouse anti-Tubulin Sigma Aldrich Cat# T5168 Mouse anti-Tubulin Sigma Aldrich Cat# T9026 Rat anti-M18BP1 Conti et al. 34 N/A Mouse anti-CyclinB1 Santa Cruz Cat# sc-245 Rabbit anti-CyclinB1 Abcam Cat# Ab32053 Mouse anti-PCNA Cell Signalling Technology Cat# 2586S Mouse anti-Histone H3 Abcam Cat# ab10799 Rabbit anti-MCPH1 Cell Signalling Technologies Cat# 4120 Rabbit anti-GFP Musacchio Lab N/A Goat anti-CENP-C Musacchio Lab N/A Bacterial and virus strains RosettaTM(DE3) Competent Cells Novagen Cat#70954 DH10EMBacY Gibco Cat#10361012 Chemicals, peptides, and recombinant proteins Human condensin I-strep Kong et al. 46 N/A Human condensin II-strep Kong et al. 46 N/A Human condensin II ΔCAP-G2 Houlard et al. 19 N/A Human condensin II Qloop-strep Kong et al. 46 N/A CAP-G2 Kong et al. 46 N/A CAP-H2 Kong et al. 46 N/A MBP-MCPH1 1-195 Houlard et al. 19 N/A MBP-MCPH1 196-435 Houlard et al. 19 N/A MBP-MCPH1 1-435 Houlard et al. 19 N/A MBP-M18BP1 873-1132 This study N/A MBP-M18BP1 873-1132-4A This study N/A MBP-M18BP1 983-1045 This study N/A MBP-M18BP1 983-1045-7A This study N/A 5FAM-MCPH1 407-422 GenScript N/A 5FAM-MCPH1 407-422 -phosphoSer417 GenScript N/A Sulfo-SDA (Sulfo-NHS-Diazirine) Sigma-Aldrich Cat803340 Cellfectin II ThermoFisher Cat# 10362100 Benzonase Sigma-Aldrich Cat# E1014 ATP Sigma-Aldrich Cat# A1852 PierceTM Protease Inhibitor Tablets, EDTA-free ThermoFisher Cat# A32965 Terrific Broth ThermoFisher Cat# 22711022 (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 17, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMP-PNP Roche Cat# 10102547001 DTT Thermo Fisher Cat# 10699530 Iodoacetamide Sigma-Aldrich I6125 Trypsin Protease Pierce 90057 Acetonitrile, LC-MS grade Sigma-Aldrich Cat# A955-4 Trifluoroacetic acid (TFA), LC-MS grade Sigma-Aldrich Cat# 302031 DMSO Thermo Fisher Cat# D2650 β-Octylglucoside (beta-glucopyranoside) Sigma-Aldrich Cat# O8001 Tween-20 Sigma-Aldrich Cat# P9416 CDK1-Cyclin B with CKS1 Huis In ’t Veld et al. 58 N/A Fugene6 Promega Cat# E2692 Nocodazole Sigma Cat# m1404 Prolong Gold Antifade Invitrogen Cat# P36930 ProLong Glass Antifade Mountant with NucBlue Invitrogen Cat# P36981 Janelia Fluor HaloTag ligand 646 Promega Cat# GA1121 Doxycycline Sigma-Aldrich Cat# D9891 Puromycin Sigma-Aldrich Cat# P9620 Thymidine Sigma-Aldrich Cat# T1895 MG132 MilliporeSigma Cat# 474790 RO-3306 Merck Millipore Cat# 217699 SNAP-Cell Block New England Biolabs (NEB) Cat# S9106S SNAP-Cell 647 New England Biolabs (NEB) Cat# S9102S Janelia Fluor HaloTag Ligand 646 Promega Cat# GA1121 Phosphatase Inhibitor Cocktail Sigma Cat# P0044 DNase I (RNase-free) Invitrogen (Ambion) Cat# AM2222 DAPI Sigma-Aldrich Cat# D9542 Mowiol EMD Millipore Cat# 475904 Triton X-100 Sigma-Aldrich Cat# T8787 NP-40 Sigma-Aldrich Cat# 74385 Opti-MEM Gibco Cat# 31985070 Critical commercial assays ATPase/GTPase Activity Assay Kit Sigma-Aldrich Cat#MAK-113 Superose 6 Increase Sigma-Aldrich Cat#GE29-0915-98 Superdex 30 Increase 10/300 GL column Cytiva GE29219757 Deposited data Atomic coordinates of the condensin II-M18BP1 complex PDB 9F5W Cryo-EM map of the condensin II-M18BP1 complex EMDB EMD-50201 Mass spec proteomics data PRIDE PXD051556 Mass spec XL-MS data PRIDE PXD051886 Genetic screen This study European Nucleotide Archive accession number PRJEB89880 Experimental models: Cell lines sf9 GIBCO Cat#11496015 High Five cells GIBCO Cat#B85502 hTERT RPE1 ATCC CRL-4000 hTERT RPE1 M18BP1-mNeonGreen-FKBP-V This study N/A HeLa CENP-A-SNAP Flp-In T-REx Pan et al. 28 N/A (Continued on next page) Molecular Cell 85, 1–13.e1–e11, July 17, 2025 e2

    Negative Control:

    Article Title: NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts.
    Article Snippet: .. Briefly, 400 ng of pPUbNSm-V5, pPUb-NSm, or pPub-Gal4 (negative control for V5 staining) per female mosquito were gently mixed with Cellfectin II (Thermo Fisher Scientific) as 1:1 ratio (vol/vol) and incubated for 20min at room temperature prior to thoracic microinjection of 1- and 2-day-old coldanaesthetised female mosquitoes using a nanoinjector (Nanoject III, Drummond Scientific). ..

    Article Title: NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts
    Article Snippet: .. Briefly, 400 ng of pPUb-NSm-V5, pPUb-NSm, or pPub-Gal4 (negative control for V5 staining) per female mosquito were gently mixed with Cellfectin II (Thermo Fisher Scientific) as 1:1 ratio (vol/vol) and incubated for 20 min at room temperature prior to thoracic microinjection of 1- and 2-day-old cold-anaesthetised female mosquitoes using a nanoinjector (Nanoject III, Drummond Scientific). ..

    Staining:

    Article Title: NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts.
    Article Snippet: .. Briefly, 400 ng of pPUbNSm-V5, pPUb-NSm, or pPub-Gal4 (negative control for V5 staining) per female mosquito were gently mixed with Cellfectin II (Thermo Fisher Scientific) as 1:1 ratio (vol/vol) and incubated for 20min at room temperature prior to thoracic microinjection of 1- and 2-day-old coldanaesthetised female mosquitoes using a nanoinjector (Nanoject III, Drummond Scientific). ..

    Article Title: NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts
    Article Snippet: .. Briefly, 400 ng of pPUb-NSm-V5, pPUb-NSm, or pPub-Gal4 (negative control for V5 staining) per female mosquito were gently mixed with Cellfectin II (Thermo Fisher Scientific) as 1:1 ratio (vol/vol) and incubated for 20 min at room temperature prior to thoracic microinjection of 1- and 2-day-old cold-anaesthetised female mosquitoes using a nanoinjector (Nanoject III, Drummond Scientific). ..

    Incubation:

    Article Title: NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts.
    Article Snippet: .. Briefly, 400 ng of pPUbNSm-V5, pPUb-NSm, or pPub-Gal4 (negative control for V5 staining) per female mosquito were gently mixed with Cellfectin II (Thermo Fisher Scientific) as 1:1 ratio (vol/vol) and incubated for 20min at room temperature prior to thoracic microinjection of 1- and 2-day-old coldanaesthetised female mosquitoes using a nanoinjector (Nanoject III, Drummond Scientific). ..

    Article Title: NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts
    Article Snippet: .. Briefly, 400 ng of pPUb-NSm-V5, pPUb-NSm, or pPub-Gal4 (negative control for V5 staining) per female mosquito were gently mixed with Cellfectin II (Thermo Fisher Scientific) as 1:1 ratio (vol/vol) and incubated for 20 min at room temperature prior to thoracic microinjection of 1- and 2-day-old cold-anaesthetised female mosquitoes using a nanoinjector (Nanoject III, Drummond Scientific). ..

    Microinjection:

    Article Title: NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts.
    Article Snippet: .. Briefly, 400 ng of pPUbNSm-V5, pPUb-NSm, or pPub-Gal4 (negative control for V5 staining) per female mosquito were gently mixed with Cellfectin II (Thermo Fisher Scientific) as 1:1 ratio (vol/vol) and incubated for 20min at room temperature prior to thoracic microinjection of 1- and 2-day-old coldanaesthetised female mosquitoes using a nanoinjector (Nanoject III, Drummond Scientific). ..

    Article Title: NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts
    Article Snippet: .. Briefly, 400 ng of pPUb-NSm-V5, pPUb-NSm, or pPub-Gal4 (negative control for V5 staining) per female mosquito were gently mixed with Cellfectin II (Thermo Fisher Scientific) as 1:1 ratio (vol/vol) and incubated for 20 min at room temperature prior to thoracic microinjection of 1- and 2-day-old cold-anaesthetised female mosquitoes using a nanoinjector (Nanoject III, Drummond Scientific). ..



    Similar Products

    cellfectin ii  (Thermo Fisher)
    90
    Thermo Fisher cellfectin ii
    Cellfectin Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellfectin+ii/cellfectin+ii/pmc10903943__Supplemental_Methods_and_References-25-14-16
    Average 90 stars, based on 1 article reviews
    cellfectin ii - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    cellfectin ii  (Fisher Scientific)
    86
    Fisher Scientific cellfectin ii
    Cellfectin Ii, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellfectin+ii/cellfectin+fisher+ii+pi20501+reagent+scientific/bio_rxiv__64898__2026__02__22__707342-46-15-29
    Average 86 stars, based on 1 article reviews
    cellfectin ii - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    cellfectin® ii cationic liposome transfection reagent  (Thermo Fisher)
    90
    Thermo Fisher cellfectin® ii cationic liposome transfection reagent
    Cellfectin® Ii Cationic Liposome Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellfectin+ii/liposome+transfection+reagent+cellfectin+ii+reagent/pm40664051-84-31-37
    Average 90 stars, based on 1 article reviews
    cellfectin® ii cationic liposome transfection reagent - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    cellfectin ii reagent  (Thermo Fisher)
    90
    Thermo Fisher cellfectin ii reagent
    Cellfectin Ii Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellfectin+ii/cellfectin+ii/pm40440302-400-13-16
    Average 90 stars, based on 1 article reviews
    cellfectin ii reagent - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    cellfectin™ ii reagent  (Thermo Fisher)
    90
    Thermo Fisher cellfectin™ ii reagent
    Cellfectin™ Ii Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellfectin+ii/cellfectin+ii/pmc12084927-105-21-24
    Average 90 stars, based on 1 article reviews
    cellfectin™ ii reagent - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    cellfectin ii thermo fisher 10362100  (Thermo Fisher)
    90
    Thermo Fisher cellfectin ii thermo fisher 10362100
    Cellfectin Ii Thermo Fisher 10362100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellfectin+ii/cellfectin+ii+thermo+fisher+10362100/pmc12038025-296-31-33
    Average 90 stars, based on 1 article reviews
    cellfectin ii thermo fisher 10362100 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    bacmid transfection agent thermofisher cellfectin® ii reagent  (Thermo Fisher)
    90
    Thermo Fisher bacmid transfection agent thermofisher cellfectin® ii reagent
    Bacmid Transfection Agent Thermofisher Cellfectin® Ii Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellfectin+ii/bacmid+transfection+agent+thermofisher+cellfectin++ii+reagent/us12281321-2265-16-19
    Average 90 stars, based on 1 article reviews
    bacmid transfection agent thermofisher cellfectin® ii reagent - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results